home

Proteomics

Storing human plasma at -30°C, rather than the -80°C recommended in current standard operating procedures, has no adverse effects on the abundances of high-molecular-weight proteins, say Spanish scientists. This will allow the analysis of far more samples by 2D-DIGE in the study of human disease.

Cuttings of <em>Populus kangdingensis</em> C. Wang et Tung and <em>Populus cathayana</em> Rehder were examined during a single growing season in a greenhouse for comparative analysis of their physiological and proteomic responses to drought stress.

A combined transcriptomic and proteomic approach has been used for the first time to construct a gene and protein data reference set for a marine fouling invertebrate, as a rapid alternative to whole genome sequencing.

The key major allergenic proteins in black tiger prawns were identified using immunoblotting and proteomics techniques, and a signature peptide for tropomyosin, the major allergen, was identified for future quantitative studies in the seafood industry.

A systematic review of multiple independent proteomic analyses of glioma combined with a network analysis of differentially expressed proteins has revealed a robust and cohesive network for glioblastoma.

A protein microarray containing almost 2000 open reading frames has been used to detect seroactive antigens for the bacterium <em>Coxiella burnetti</em>, the agent of Q fever. Some of the antigens were used in an immunostrip for disease diagnosis and are candidates for vaccines.

The changes in protein expression in an embryonic snail as it develops from a shell-less to a shelled form have been tracked by Taiwanese scientists, using de novo peptide sequencing to counter the lack of gastropod genomic and proteomic data.

The interactions of Cr(III) complexes with serum proteins and their internalization by U937 macrophage-like cells was studied by a proteomic approach, in the context of Co-Cr alloy-based implants.

The plasma proteomes of patients with glioblastoma multiforme brain tumours were used to classify the type of tumour and indicate which patients would respond best to immunotherapy, heralding a new approach to personal medicine.

A detailed study of the urinary proteome following a period of endurance exercise revealed several proteins at increased levels, which were linked to physiological changes. The results form the basis of a plan to establish individual 2D PAGE protein maps which could lead to a personal monitoring system for athletes.

Real-life male and female urine samples with well-characterized proteomes and peptidomes have been generated for use as reference standards in urine clinical proteomics studies.

A study of transgenic maize plants using several -omic techniques revealed that the local growing environment had a far greater effect on gene expression, protein and metabolite levels than variations in genotypes.

MALDI mass spectrometry has revealed that cells in the apparently normal tissue left behind following the surgical removal of a tumour bear a close resemblance to tumour cells at the molecular level. This might explain why so many cancers recur and suggests that tumour margins should be extended for maximum patient safety.

Progress in understanding root growth regulation and adaptation under water-stressed conditions is reviewed, with emphasis on recent advances from transcriptomic and proteomic analyses of maize and soybean primary roots.

A proteomics study of ovarian cancer has identified a series of proteins that might be implicated in multidrug resistance to cisplatin, a drug commonly used for treatment. The involvement of two proteins in particular was shown to be highly probable.

Keloid scar tissue formed on human skin contains altered levels of proteins related to inflammation, fibrosis, antifibrosis and tumour suppression, say researchers in Singapore, some of which might be useful targets for controlling scar formation.

The anatomy and function of the circadian-generating system in mammals is described, and recent proteomic studies that investigate day/night changes in the retina, suprachiasmatic nucleus and pineal gland are reviewed.

A new protocol for the high-throughput proteomics analysis of intact proteins has been demonstrated by scientists in the USA, who combined pre-fractionation and mass spectrometry with high resolution tandem mass spectrometry to allow the identification of proteins up to 80 kDa.

A long-term study combining proteomic and brain imaging technologies has identified one particular protein as a marker of brain injury in people in the advanced stages of HIV infection, a condition which is difficult to track as it is normally clinically silent.

The available literature on proteomic experiments that analyze human cerebrospinal fluid (CSF) and brain extracellular fluid (ECF), mostly obtained by cerebral microdialysis, has been reviewed.