HPLC
Moderate heat for fast RPLC Fast separations have been achieved on particulate and monolithic columns with conventional HPLC at intermediate temperatures, as evidenced by the analysis of two classes of pharmaceuticals which were separated within 2 minutes.
Liquid chromatography on a chip This review emphasizes the development of novel stationary phases and their implementation in microchannels in lab-on-a-chip systems with reference to recent instrumental advances.
Is there any DNA there? The presence of DNA in ancient bone specimens can be inferred from the extent of amino acid racemisation, say Italian scientists, who used HPLC with fluorescence detection to measure the D/L ratios of alanine and aspartic acid.
Antioxidants attacked The antioxidant levels in complex plant matrices have been determined by two HPLC methods employing post-column addition of reagents. The first involved a cupric ion reagent that is reduced by the antioxidants whereas the second is an improved and refined method based on the DPPH radical.
A RP-HPLC-DAD-APCI/MSD method for the characterisation of medicinal Ericaceae used by the Eeyou Istchee Cree First Nations An HPLC-DAD-APCI/MSD method was developed to detect phenolic compounds in the leaf extracts of 14 Ericaceae plants used by indigenous North Americans to treat degenerative diseases including type 2 diabetes.
Raising expectations The illicit presence of four synthetic prescription drugs in dietary supplements intended to improve male sexual potency has been determined by Chinese scientists using a simple but effective HPLC/MS procedure valid down to 1 ppm.
Leafy monitors Mangrove and medlar leaves have been used as passive samplers to measure local concentrations of PAHs by HPLC with fluorescence detection. The miniaturised method effectively provides an infinite number of sampling points for assessing local and regional atmospheric contamination.
Liquid chromatography-tandem mass spectrometry applications in endocrinology This review focuses on the use of LC-MS/MS in clinical laboratories to assist with the diagnosis of diverse groups of endocrine and metabolic diseases.
HILIC beats ion pairing for aminoglycosides HILIC performed far better than ion pairing HPLC in conjunction with mass spectrometric detection for the analysis of two aminoglycoside antibiotics in milk, say Greek researchers, improving the sensitivity by at least 80-fold.
Screaming yellow The flavonoids in yellow lake pigments and some of the related plants from which they are derived have been identified by LC/MS, permitting the plant provenances of pigments in <em>The Scream</em> and the <em>Reclining Tiger</em> to be attributed.
Analysis of ascorbic acid based black powder substitutes by HPLC/electrospray ionization quadrupole time-of-flight mass spectrometry An HPLC-electrospray ionization-QTOF MS method that fulfills the degree of certainty required in a court of law has been developed for the analysis of both the organic and inorganic constituents of black powder substitutes.
Antibiotics clarified in grainy picture A single HPLC/MS method has been developed for measuring a set of 13 antibiotics from several classes in distillers grain, a by-product of the ethanol fuel industry bound for use as an animal feed.
Ice-cold pollution record The historical record of PAH pollution in Europe over the past 300 years has been derived from ice cores drilled from the Alps, using a sequential melting system and HPLC measurement.
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Foiling fuel fraud HPLC with electrochemical detection and minimal sample preparation have been used to determine three marker dyes in fuels, which are added for identification and to prevent fraud.
Colourless flavonoids in pollen A series of colourless flavonoids have been identified in the bee pollen from a common weed using LC/MS combined with photodiode array detection, which will be useful for the quality control of dietary supplements based on this product.
HPLC method validation for measurement of sulforaphane level in broccoli by-products A simple and specific analytical for the determination of sulforaphane in broccoli by-products includes optimization of the conversion of glucoraphanin to sulforaphane.
A sequence optimization strategy for chromatographic separation in RP-HPLC A sequence optimization strategy combining an artificial neural network and a chromatographic response function for chromatographic separation in reversed-phase high-performance liquid chromatography has been proposed.
The fifth flavour quantified Five stationary phases have been compared for the HPLC separation of nucleosides and mononucleotides without using ion pairing, to help determine the umami factors in a series of edible wild mushrooms.
Cheating cheese The amount of sheep's milk fraudulently present in cheeses made primarily from cows' or goats' milk can be estimated by liquid chromatography-tandem mass spectrometry of casein fragments, say Italian researchers.