Electrophoresis
Proteome approaches combined with FT-IR spectroscopy revealed a distinctive biofilm physiology in <i>Bordetella pertussis</i> Using 2D gel electrophoresis and Fourier transform infrared spectroscopy, an international team of scientists has investigated the physiology of mature biofilm in <i>Bordetella pertussis</i>, the agent responsible for whooping cough.
CE probes biochemistry Two US chemists have come up with a way to combine microdialysis, capillary electrophoresis and amperometric detection onto a single microchip, and are now using it to probe the biochemistry of Parkinson's disease.
Identification of inorganic ions in post-blast explosive residues using portable CE instrumentation Australian researchers have developed novel capillary electrophoresis methods on portable instruments for separating and detecting inorganic anions and cations in post-blast residues from homemade inorganic explosive devices.
Determination of pathogenic bacteria by CZE with surface-modified capillaries Polish chemists have developed a novel capillary electrophoresis method for determining pathogenic bacteria in clinical samples, especially those that cause serious illnesses in hospitals.
Pinpointing the plaque proteins French and German scientists have developed a capillary electrophoresis method for separating five almost identical beta-amyloid proteins, which clump together to form the plaques that are hallmarks of Alzheimer's disease.
Boronate affinity saccharide electrophoresis: A novel carbohydrate analysis tool British scientists have developed a novel electrophoresis method for analysing sugar-containing molecules by incorporating specialised carbohydrate affinity ligand methacrylamido phenylboronic acid in polyacrylamide gels.
Heat induced changes in protein expression profiles of Norway spruce ecotypes from different elevations By analysing changes in protein expression using 2-DE, German and French scientists have revealed that strains of Norway spruce adapted to different elevations vary in their ability to handle large movements in temperature.
You don't have to be mad to work here Using two-dimensional gel electrophoresis and mass spectrometry, Canadian and German scientists have discovered that a single protein can accurately distinguish between healthy cows and those with mad cow disease.
Applications of CE SDS gel in development of biopharmaceutical antibody-based products US bioscientists have shown how the capillary electrophoresis SDS gel technique can support the development of antibody-based products from early clone screening for product quality to final product characterisation.
A proteomic characterization of water buffalo milk fractions Italian analytical scientists have studied the protein content of water buffalo milk using gel electrophoresis and mass spectrometry, in order to investigate using the milk to manufacture high-quality dairy products.
All in the mix By taking advantage of the first effective method for mixing together different reactants within a capillary tube, an international team of chemists has come up with a totally novel method for screening enzyme inhibitors.
Bilayer disk capillary electrophoresis: A novel method to study drug partitioning into membranes Swedish researchers have developed a new form of capillary electrophoresis, which involves using lipid bilayer discs as a pseudostationary phase, and shown that it can be used as a realistic model for cell membranes.
Proteomic analysis of larvae during development, attachment, and metamorphosis in the fouling barnacle To clarify the overall profile of protein expression during larval development and metamorphosis of the barnacle, Chinese biologists have used two dimensional gel electrophoresis to study its proteome at different life stages.
Wave goodbye to mechanical pumps By separating analytes as waves, Dutch nanotechnologies have managed to ditch the need for a mechanical pump in the continuous separation method known as free-flow electrophoresis.
Development of a temperature gradient focusing method for <i>in situ</i> extraterrestrial biomarker analysis By applying scanning temperature gradient focusing to the chiral separation of amino acids, US biochemists have shown that it could be a viable method for analysing biomarkers in future missions to Mars or other solar system bodies.
Proteomic investigation of amino acid catabolism in the indigenous gut anaerobe Fusobacterium varium Using 2D gel electrophoresis, high performance liquid chromatography and tandem mass spectrometry, Canadian scientists have investigated amino acid fermentation in the gut microbe Fusobacterium varium.
NO lighting up By employing a novel fluorescent probe, Chinese researchers have shown that capillary electrophoresis can be an ideal method for studying the release of the gaseous signalling molecule nitric oxide from single cells.
Photopatterning of tough CNT composites in microfluidic channels and their application in gel-free separations Taiwanese chemists have combined single-walled carbon nanotubes with soft hydrogel polymers to create a strong nanocomposite hydrogel that can concentrate and separate proteins in glass microchannels within 1.5cm.
A practical interface for microfluidics and nanoelectrospray mass spectrometry Canadian researchers have developed a new method for fabricating nanospray ionization tips for mass spectrometry, formed from glass substrates and the inert polymer, parylene-C.
Cut glass acoustics Sound waves can transport particles just as well in glass chips as in silicon chips, say Swedish researchers, potentially leading to acoustophoresis becoming much more widely used for separation purposes.