Electrophoresis
Analysis of pressure ulcer wound fluid using two-dimensional electrophoresis US scientists have used 2D gel electrophoresis to analyse the proteins in pressure ulcer wound fluid, in order to find early indicators and biomarkers for this condition.
Quick arm of the law While-you-wait DNA profiles should soon be available for anyone arrested by the police, thanks to a new microfluidic device developed by British and US scientists.
Synthesis of hydroxyethylcellulose-<i>graft</i>-poly(<i>N, N</i>-dimethylacrylamide) copolymer by ATRP and as dynamic coating in CE Chinese material scientists have developed a new dynamic coating for capillary electrophoresis, which prevents proteins sticking to capillary walls.
Identification of inflammation-related proteins in a murine colitis model by 2D fluorescence difference gel electrophoresis and mass spectrometry In order to discover more about the acute inflammation caused by ulcerative colitis, Japanese scientists used 2D fluorescence difference gel electrophoresis to analyse the proteins expressed in the intestines of mice.
Follow the light To control the movement of individual droplets and their molecular contents, all you need are different size beams of light, say US scientists.
Plasma protein electrophoresis of <i>Trachemys scripta</i> and <i>Iguana iguana</i> Using two different forms of gel electrophoresis, Spanish scientists have analysed the proteomes of two species of reptile: a turtle and an iguana.
Post-translation modification of proteins in tears Australian scientists have conducted the first 2D gel electrophoresis study using sequential dyes to analyse phospho-, glyco- and total tear protein profiles, discovering a novel tear protein in the process.
Chasing comets A team of US scientists has developed a method for locating exactly where a comet will be at any given point, but only if it's made of DNA.
The effect of weight loss on protein profiles of gastrocnemius muscle in rabbits Using gel electrophoresis and peptide mass fingerprinting, Portuguese scientists investigated the effects of food restriction on gastrocnemius muscle protein expression in New Zealand White (meat producing) and wild rabbits.
Microchip electrophoretic separation for the fast diagnosis of <i>Anaplasma phagocytophilum</i> infection in cattle Using PCR and microchip electrophoresis, Korean scientists have developed a novel method for diagnosing cattle infected with the bacterium <i>Anaplasma phagocytophilum</i>, which can also cause disease in humans.
Another brick in the wall Self-assembling nanoparticle 'walls' are just as good at separating DNA strands as polymer gels, say Canadian scientists, but for the best results the gaps in the walls should be as small as possible.
Electrophoresis today and tomorrow: Helping biologists' dreams come true This review looks at the various electrophoresis techniques that are available for studying biomolecules like proteins, including 2D electrophoresis, isoelectric focusing, isotachophoresis and microchip electrophoresis.
Creation of arrays of cell aggregates in defined patterns for developmental biology studies using dielectrophoresis British scientists have shown that dielectrophoresis - the movement of particles in non-uniform electric fields - can be used to create engineered skin with artificial placodes of different sizes and shapes, in different spatial patterns.
We are all individuals Conventional DNA fingerprinting can distinguish between individuals and identify family ties, but now US scientists have produced a new version that can also accurately determine gender and ethnicity.
Focusing of anionic micelles using sample-induced transient isotachophoresis An international team of researchers has shown that transient isotachophoresis can be used to focus anionic micelles prior to micellar electrokinetic chromatography.
A novel polyacrylamide gel system for proteomic use offering controllable pore expansion by crosslinker cleavage To make it easier to extract proteins after 2D gel electrophoreis, German scientists have developed a novel partly-reversible gel system, in which the gel matrix can be conditionally cleaved to increase the pore diameters.
Cometh the hour, cometh the DNA By shifting the balance of power between electrophoresis and electro-osmosis, Dutch and US chemists have managed to concentrate DNA into bands just 10µm wide.
Fish proteome analysis: Model organisms and non-sequenced species This review describes proteomic approaches, such as gel electrophoresis, that have been used to investigate diverse biological questions in model and non-model fish species, incuding questions about physiology and developmental biology.
Lectin affinity electrophoresis of serum alkaline phosphatase in metastasized breast cancer Using lectin affinity electrophoresis, French scientists have investigated the use of serum alkaline phosphatase isoenzymes as markers of breast cancer.
If you can't stand the heat The migration of particles along a temperature gradient, known as thermophoresis, could allow biomolecules to be analysed entirely within biological fluids such as blood serum, say German physicists.